ABSTRACT
Objective: To investigate the chemical constitutes of Bombyx Batryticatus. Methods: The compounds were isolated by means of solvent extraction, repeated silica gel column chromatography, ODS, and preparative RP-HPLC. The structures were identified by spectral analysis and chemical evidence. Results: Four compounds of steroids were isolated from the Bombyx Batryticatus and were identified as (3α,6β)-3-benzyl-6-isopropyl-4-methyl-2,5-morpholinedione (1), (3α,6α)-3-benzyl-6-isopropyl- 4-methyl-2,5-morpholinedione (2), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (3), 22E-3β-hydroxy-5α,6α-epoxyergosta-22-en-7-one (4), 5α,6α-epoxy-(22E,24R)-ergosta-8(14),22-diene-3β,7α-diol (5), 7α-methoxy-(22E,24R)-5α,6α-epoxyergosta-8(14),22-diene-3β-ol (6), (22E,24R)-ergosta-5,7,22-trien-3β-ol (7), stigmasta-7,22-diene-3β,5α,6α-triol (8), 3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-diene-6-one (9), (22E,24S)-5α,8α-epidioxy-24-methylcholesta-6,9(11),22-trien-3β-ol (10), homoarginine (11), and β-sitosterol (12). Conclusion: Compounds 1-11 are isolated from Bombyx Batryticatus for the first time.